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Image Search Results
Journal: Acta Biochimica et Biophysica Sinica
Article Title: 8-Oxoguanine DNA glycosylase protects cells from senescence via the p53-p21 pathway
doi: 10.3724/abbs.2023264
Figure Lengend Snippet: OGG1 modulates cell transformation in A549 cells in the presence of BLM A549 cells were transfected with siRNA-NC or siOGG1 (A,C), VC or OGG1 (B,D) 24 h and then treated with 25 μg/mL BLM for 24 h. EMT markers, including FN1, N-cadherin and α-SMA proteins, were detected by western blot analysis. *P<0.05 vs NC or VC; #P<0.05 vs NC+BLM or VC+BLM. Data are expressed as the mean±SD of the experiments performed in triplicate.
Article Snippet: The following primary antibodies were used: OGG1 (ab124741; Abcam, Cambridge, UK), NB100-106 (Novus Biologicals), telomerase reverse transcriptase (TERT) (ab32020; Abcam), Lamin B1 (17416S), p53 (2425S), p-p53 (9284S), p21 (2947S), p-H2AX (9718S), N-cadherin (13116S) (Cell Signaling, Danvers, USA),
Techniques: Transformation Assay, Transfection, Western Blot